4 research outputs found
Isolation and characterization of new Puumala orthohantavirus strains from Germany
Orthohantaviruses are re-emerging rodent-borne pathogens distributed all over the world. Here, we report the isolation of a Puumala orthohantavirus (PUUV) strain from bank voles caught in a highly endemic region around the city OsnabrĂĽck, north-west Germany. Coding and non-coding sequences of all three segments (S, M, and L) were determined from original lung tissue, after isolation and after additional passaging in VeroE6 cells and a bank vole-derived kidney cell line. Different single amino acid substitutions were observed in the RNA-dependent RNA polymerase (RdRP) of the two stable PUUV isolates. The PUUV strain from VeroE6 cells showed a lower titer when propagated on bank vole cells compared to VeroE6 cells. Additionally, glycoprotein precursor (GPC)-derived virus-like particles of a German PUUV sequence allowed the generation of monoclonal antibodies that allowed the reliable detection of the isolated PUUV strain in the immunofluorescence assay. In conclusion, this is the first isolation of a PUUV strain from Central Europe and the generation of glycoprotein-specific monoclonal antibodies for this PUUV isolate. The obtained virus isolate and GPC-specific antibodies are instrumental tools for future reservoir host studies
Cluster of human Puumala orthohantavirus infections due to indoor exposure?—An interdisciplinary outbreak investigation
Puumala orthohantavirus (PUUV) is the most important hantavirus species in Europe, causing the majority of human hantavirus disease cases. In central and western Europe, the occurrence of human infections is mainly driven by bank vole population dynamics influenced by beech mast. In Germany, hantavirus epidemic years are observed in 2- to 5-year intervals. Many of the human infections are recorded in summer and early autumn, coinciding with peaks in bank vole populations. Here, we describe a molecular epidemiological investigation in a small company with eight employees of whom five contracted hantavirus infections in late 2017. Standardized interviews with employees were conducted to assess the circumstances under which the disease cluster occurred, how the employees were exposed and which counteractive measures were taken. Initially, two employees were admitted to hospital and serologically diagnosed with hantavirus infection. Subsequently, further investigations were conducted. By means of a self-administered questionnaire, three additional symptomatic cases could be identified. The hospital patients' sera were investigated and revealed in one patient a partial PUUV L segment sequence, which was identical to PUUV sequences from several bank voles collected in close proximity to company buildings. This investigation highlights the importance of a One Health approach that combines efforts from human and veterinary medicine, ecology and public health to reveal the origin of hantavirus disease clusters.Peer Reviewe
Recent Experimental Tests of Special Relativity
We review our recent Michelson-Morley (MM) and Kennedy-Thorndike (KT)
experiment, which tests Lorentz invariance in the photon sector, and report
first results of our ongoing atomic clock test of Lorentz invariance in the
matter sector. The MM-KT experiment compares a cryogenic microwave resonator to
a hydrogen maser, and has set the most stringent limit on a number of
parameters in alternative theories to special relativity. We also report first
results of a test of Lorentz invariance in the SME (Standard Model Extension)
matter sector, using Zeeman transitions in a laser cooled Cs atomic fountain
clock. We describe the experiment together with the theoretical model and
analysis. Recent experimental results are presented and we give a first
estimate of components of the parameters of the SME matter
sector. A full analysis of systematic effects is still in progress, and will be
the subject of a future publication together with our final results. If
confirmed, the present limits would correspond to first ever measurements of
some components, and improvements by 11 and 14 orders of
magnitude on others.Comment: 29 pages. Contribution to Springer Lecture Notes, "Special Relativity
- Will it survive the next 100 years ?", Proceedings, Potsdam, 200
Testing General Relativity with Atomic Clocks
We discuss perspectives for new tests of general relativity which are based
on recent technological developments as well as new ideas. We focus our
attention on tests performed with atomic clocks and do not repeat arguments
present in the other contributions to the present volume. In particular, we
present the scientific motivations of the space projects ACES and SAGAS.Comment: Contribution for "The Nature of Gravity" (eds. F. Everitt et al